质粒名称: | pENTR-GFP-MDC1 (770-7) |
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出品公司: | 爱迪基因 |
目录编号: | 26284 |
存储实验室: | Eric Campeau |
载体骨架基本信息 |
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载体名称: | pENTR4 |
载体出品公司: | Invitrogen |
载体抗性: | 卡那霉素 |
空载体大小: | 3270 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | Entry vector |
筛选标记: | -- |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | Mediator of DNA damage checkpoint 1,MDC1,NFBD1,KIAA0170 |
物种来源: | H. sapiens (human) |
插入大小: | 6269 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子 | CMV |
标签/融合蛋白: | GFP(主干上的N终端) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | Bst BI(未破坏) |
3' 克隆位点: | Xba I (未破坏) |
5' 测序引物 | ENTRforw |
3' 测序引物 | ENTRrev |
文献引用方法 |
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材料和方法部分: | pENTR-GFP-MDC1 (770-7) was a gift from Eric Campeau (Addgene plasmid # 26284 ; http://n2t.net/addgene:26284 ; RRID:Addgene_26284) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pENTR-GFP-MDC1 (770-7) 9539 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pENTR-GFP-MDC1 (770-7) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9539) TITLE New vectors Aug. 2010 REFERENCE 2 (bases 1 to 9539) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9539 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 103..189 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 281..308 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" primer_bind 316..339 /label=pENTR-F /note="pENTR vectors, forward primer" protein_bind 358..457 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" regulatory 498..507 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 504..1220 /codon_start=1 /product="enhanced GFP" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(549..570) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(810..829) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1157..1178 /label=EGFP-C /note="EGFP, forward primer" protein_bind complement(7768..7867) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(7979..7998) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 7990..8799 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(8057..8076) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 8889..9477 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9378..9397 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 421 ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatgggaac caattcagtc 481 gactagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg 541 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 601 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 661 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca 721 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct 781 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 841 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 901 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 961 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 1021 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 1081 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca 1141 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 1201 gcatggacga gctgtacaag tccggccgga ctcagatctc gagctcaagc ttcgaattct 1261 gcagtcgacg gtaccgcggg cccatcatgg aggacaccca ggctattgac tgggatgttg 1321 aagaagagga ggagacagag caatccagtg aatccttgag gtgtaacgtg gagccagtag 1381 ggcggctaca tatctttagt ggtgcccatg gaccagaaaa agatttccca ctacacctcg 1441 ggaagaatgt ggtaggccga atgcctgact gctctgtggc cctgcccttt ccatctatct 1501 ccaaacaaca tgcagagatt gaaatcttag cctgggacaa ggcacctatc ctccgagact 1561 gtgggagcct taatggtact caaatcctga gacctcctaa ggttttgagc cctggggtga 1621 gtcaccgtct gagggaccag gaattgattc tctttgctga cttgctctgc cagtaccatc 1681 gcctggatgt ctctctgccc tttgtctccc ggggccctct gacagtagaa gagacaccca 1741 gagtacaggg agaaactcaa ccccagaggc ttctgttggc tgaggactcg gaggaggaag 1801 tagattttct ttctgaaagg cgtatggtaa aaaaatcaag gaccacatct tcctctgtga 1861 tagttccaga gagtgatgaa gaggggcatt ccccggtcct gggcggcctt gggccgcctt 1921 ttgccttcaa tttgaacagt gacacagatg tggaagaagg tcagcaacca gccacagagg 1981 aggcctcctc agctgccaga agaggtgcca ctgtagaggc aaagcagtct gaagctgaag 2041 ttgtaactga aatccagctt gaaaaggatc agcctttagt gaaggagagg gacaatgata 2101 caaaagtcaa gaggggtgca gggaatgggg tggttccagc tggggtgatt ctggagagga 2161 gccaacctcc tggagaggac agtgacacag atgtggatga tgacagcagg cctcctggaa 2221 ggccagctga ggtccatttg gaaagggctc agccttttgg cttcatcgac agcgacactg 2281 atgcggaaga agagaggatc ccagcaaccc cagttgtcat tcctatgaag aagaggaaga 2341 tcttccatgg agtaggtaca aggggtcctg gagcaccagg cctggcccat ctgcaggaga 2401 gccaggctgg tagtgataca gatgtggaag aaggcaaggc cccacaggct gtccctctgg 2461 agaaaagcca agcttccatg gttatcaaca gcgatacaga tgacgaggaa gaagtctcag 2521 cagcgctgac tttggcacat ctgaaagaga gccagcctgc tatatggaac agagatgcag 2581 aagaggacat gccccaacgt gtggtccttc tgcagcgaag ccaaaccacc actgagagag 2641 acagtgacac agacgtggag gaggaagagc tcccagtgga aaatagagaa gctgtcctca 2701 aggatcacac aaagattaga gcccttgtta gagcacattc agaaaaggac caacctcctt 2761 ttggggacag tgatgacagt gtggaagcag ataagagctc acctgggatc cacctggaga 2821 gaagccaagc ctccaccaca gtggacatca acacacaagt ggagaaggaa gtcccgccag 2881 ggtcagccat tatgcatata aagaagcatc aggtgtctgt ggaggggaca aatcaaacag 2941 atgtgaaagc agttggggga ccagcaaagc tgcttgtggt atctctagag gaagcctggc 3001 ctctgcatgg ggactgtgaa acagatgcag aggagggcac ctccctaaca gcctcagtag 3061 ttgcagatgt aagaaagagc cagcttccag cagaagggga tgctggggca gagtgggctg 3121 cagctgttct taagcaggag agagctcatg aggtgggggc ccagggtggg ccacctgtgg 3181 cacaagtgga gcaggacctc cctatctcaa gagagaacct cacagatctg gtggtggaca 3241 cagacactct aggggaatcc acccagccac agagagaggg agcccaggtc cccacaggaa 3301 gggagagaga acaacatgtg ggtgggacca aggactctga agacaactat ggtgattctg 3361 aagatctgga cctacaagct acccagtgct ttctggagaa tcagggcctg gaagcagtcc 3421 agagcatgga ggatgaacct acccaggcct tcatgttgac tccaccccaa gagcttggcc 3481 cttcccattg cagcttccag acaacaggta ccctagatga accatgggag gtcctggcta 3541 cacagccatt ctgtctgaga gagtctgagg actctgagac ccagcctttt gacacgcacc 3601 ttgaggccta tggaccttgc ctgtctccac ctagggcaat accaggagac caacatccag 3661 agagcccagt tcacacagag ccaatgggga ttcaaggcag agggaggcag actgtggata 3721 aagtcatggg tataccaaaa gaaacagcag agagggtggg ccctgagaga gggccattgg 3781 agagagaaac tgagaaactg ctaccagaaa gacagacaga tgtgacagga gaggaagaat 3841 taaccaaggg gaaacaggac agagaacaaa aacagttgtt agctagagac acccagagac 3901 aagaatctga caaaaatggg gaaagtgcaa gtcctgaaag agatagggag agtttgaagg 3961 tagaaattga gacatctgag gaaatacaag agaaacaagt acagaagcag acccttccaa 4021 gcaaagcatt tgagagagaa gtagagagac cagtagcaaa cagagagtgc gatccagccg 4081 agttagaaga gaaggtgccc aaagtgatcc tggagagaga tacacagaga ggggagccag 4141 agggagggag ccaggaccag aaagggcagg cctccagccc aacaccagag cctggggtgg 4201 gggcggggga ccttccggga cctacctcag cccccgtacc ttctgggagc cagtcaggtg 4261 gaaggggatc cccagtgagc cccaggaggc atcagaaagg cctcctgaat tgcaagatgc 4321 cacctgctga gaaggcttcc aggatcagag ctgctgagaa ggtttccagg ggcgatcagg 4381 aatctccaga tgcttgtctg cctcctgcag tacctgaagc cccagcccca ccccaaaagc 4441 cccttaactc tcagagccag aaacatcttg cacctccgcc ccttctttct ccccttttac 4501 cttctatcaa gccaaccgtt cgtaagacca ggcaagatgg gagtcaggaa gctccagagg 4561 ctcccttgtc ctcagagctg gagcctttcc acccaaagcc taaaattaga actcggaagt 4621 cctccagaat gacacccttt ccagctacct ctgctgcccc tgagccccac ccttccacct 4681 ccacagccca gccagtcact cccaagccca catctcaggc cactaggagc aggacaaata 4741 ggtcctctgt caagacccct gaaccagttg tccccacagc ccctgagctc cagccttcca 4801 cctccacaga ccagcctgtc acctctgagc ccacatctca ggttactagg ggaagaaaaa 4861 gtagatcctc tgtcaagacc cctgaaacag ttgtgcccac agcccttgag ctccagcctt 4921 ccacctccac cgaccgacct gtcacctctg aacccacctc tcaggctact aggggaagaa 4981 aaaatagatc ctctgtcaag acccctgaac cagttgtccc cacagcccct gagctccagc 5041 cttccacctc cacagaccag cctgtcactt ctgagcccac atatcaggct actaggggaa 5101 gaaaaaatag atcctctgtc aagacccctg aaccagttgt gcccacagcc cctgagctcc 5161 ggccttccac ctccacagac cgacctgtca cccccaagcc cacatctcgg accactagga 5221 gcaggacaaa tatgtcctct gtcaagaccc ctgaaacagt tgtccccaca gcccctgagc 5281 tccagatttc cacctccaca gaccaacctg tcacccctaa gcccacatct cggaccacta 5341 ggagcaggac aaatatgtcc tctgtgaaga accctgaatc aactgtccct atagcccctg 5401 agctcccacc ttccacctcc acagagcagc ctgtcacccc tgagcccaca tctcgggcta 5461 ctaggggaag aaaaaataga tcctctggca agacccctga aacacttgtc cccacagccc 5521 ctaagctcga gccttccact tccacagacc aacctgtcac tcctgagccc acatctcagg 5581 ccaccagggg caggacaaat aggtcctctg tgaagacccc tgaaacagtt gtccccacag 5641 cccctgagct ccagccttcc acctccacag accagcctgt tacccctgag cctacgtctc 5701 aggctactag gggaagaaca gatagatcct ctgtcaagac tcctgaaaca gttgtcccca 5761 cagcccctga gctacaggct tccgcctcca cagaccagcc tgtcacctct gagcccacat 5821 ctcggaccac taggggaaga aaaaatcggt cctctgtcaa gacccctgaa acagttgtgc 5881 ccgcagcccc tgagctccag cctcccacct ccacagaccg acctgtcacc cctgagccca 5941 catctcgggc cactaggggc aggacaaata ggtcctctgt caagacccct gaatcaattg 6001 tccctatagc ccctgagctt cagccttcca cctccagaaa ccagcttgtc acccctgagc 6061 ccacatctcg ggccactagg tgcaggacaa ataggtcctc tgtcaagacc cctgagccag 6121 ttgtccccac agcccctgag ccccatccta ccacctccac agaccagcct gtcaccccca 6181 agctcacatc tagggccact aggagaaaga caaataggtc ctctgtcaag actcccaaac 6241 cagttgaacc agcagcctct gatcttgagc cttttacccc cacagaccag tccgtcaccc 6301 ctgaggccat agctcagggt ggtcagagca aaacactgag gtcttccaca gtaagagcta 6361 tgccggttcc taccacccct gaattccaat ctcctgtcac cacagaccag cctatttccc 6421 ctgagcctat tactcaaccc agttgcatca agaggcagag agccgctggg aaccctggct 6481 ccctcgcagc tcccattgac cataagcctt gctctgcacc cttggaacct aaatcccagg 6541 cctcaaggaa ccaaagatgg ggagcagtga gagcagctga atcccttaca gccattcctg 6601 agcctgcctc tccccagctt cttgagacac caattcatgc ctcccagatc caaaaggtgg 6661 aaccagcagg tagatctagg ttcaccccgg agctccagcc taaggcctct caaagccgca 6721 agaggtcttt agctaccatg gattcaccac cacatcaaaa acagccccaa agaggggaag 6781 tctcccagaa gacagtgatt atcaaggaag aggaagaaga tactgcagag aagccaggga 6841 aggaagagga tgtcgtgact ccaaaaccag gcaagagaaa gagagaccag gcagaggagg 6901 agcccaacag aataccaagc cgcagcctcc gacggaccaa acttaaccaa gaatcaacag 6961 cccccaaagt gctcttcaca ggagtggtgg atgctcgggg agagcgggct gtgctggcac 7021 tggggggaag tctggctggt tcagcggcag aggcttccca cctggtcact gatcgcatcc 7081 gccggacagt caagttcctg tgtgccctgg ggcggggaat ccccattctg tccctggact 7141 ggctgcatca gtcccgcaag gctggtttct tcttaccccc ggatgaatat gtggtgaccg 7201 accctgagca agagaagaac tttggcttta gccttcaaga cgcactgagc agggctcggg 7261 agcgaaggct gctagagggc tatgagatct atgtgacccc tggagtccag ccaccaccac 7321 ctcagatggg agagattatt agctgctgtg gaggcacata cctacccagc atgcctcggt 7381 cctataagcc tcagagagtt gtgatcacat gccctcagga cttccctcat tgctccattc 7441 cactacgggt tgggctgccc ctcctctcgc ctgagttcct gctgactgga gtgctgaagc 7501 aggaagccaa gccagaggcc tttgtcctct cccctttgga gatgtcatcc acctgagaac 7561 tccactaccc ttttccctcc cagaccacga attagaagat atgtggaaga aagaactcag 7621 ggcgttagaa aggattgggg tatattgata caacttgtcc tggaacatgg gtgggaccag 7681 aaatctttat gaataaatga aaagataagg gatttggaag ccacaggttg ttttttgttt 7741 gtttgtttgt ttttttaatg gatctagacc cagctttctt gtacaaagtt ggcattataa 7801 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca 7861 ttatttgcca tccagctgca gctctggccc gtgtctcaaa atctctgatg ttacattgca 7921 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca 7981 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg 8041 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca 8101 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt 8161 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg 8221 cctcttccga ccatcaagca ttttatccgt actcctggtg atgcatggtt actcaccact 8281 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat 8341 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt 8401 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt 8461 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg 8521 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc 8581 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga 8641 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt 8701 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat 8761 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg 8821 taacattatt cagattgggc cccgttccac tgagcgtcag accccgtaga aaagatcaaa 8881 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 8941 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 9001 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 9061 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 9121 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 9181 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 9241 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 9301 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 9361 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 9421 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 9481 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtt //